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Previous Versions

Previous Versions

Space Ranger 4.0.1 (Jun 12, 2025)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 885 MB
    md5sum: b0beb100947717cee8c7ad0d7a9f0247
    curl -o spaceranger-4.0.1.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-4.0.1.tar.gz?Expires=1770486664&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=LBlJQdumAPLXMzzITZqsVgjM-1RD48WIApmmJXZw4OenSF-XH-OGwO8EaOv7Upj-z9Th-~BOHlEkXxlQqMLQfJy5f99zb3pYlWtLkZudJRq3Dc0yWBsvbEK5Mz-kGD4R17OPWVbvVfTnCY2mn~kxOe8KSOCUbqukhf1Kkmms6zKG7MhLP5nvazkgz~yfrqwY-pI9QANJcBZ6WWIf2IyPJDpA9UxKbcHwZ89HgrH~xViHtgWPdKN9il9jp4xQVnTLtcm~D2SBzHg3TpLRexIkC7dWM7o~LFQmAqC59noK8yG88CL~m~5KruC0M2oQXwCjg4vTRXuoJztCV1v5t0zB7A__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 582 MB
    md5sum: 3610ff948a6f8129cf49509f1bb34970
    curl -o spaceranger-4.0.1.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-4.0.1.tar.xz?Expires=1770486664&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=j3ZzsHRL5mNNcAXKH8~apS6hcmDekPSn~O0A4NNQneIiaKoMgUDFI41iC4vVoZIRrBqnEK6N1xHAW~6lI9sGEm5sGhhaEIKR66V8swW2w-hAf0IM5gDCo2mD8jV-x85GRYMq3udQ~46RT75s6MJN5HLClgTNp5khYh5TGPZLowxnbbnG45wmQvv2~GCn6d3Q~jLcpua8ObDtvbIKhOaJHMMvGg9KjWXEsLx0QplnZUXJpelGu3DEV6-rwRMy9BkjFF7P32u-vLq7eqVX4DHFhqAnxdpGYHuTm4eninNmqLtAdREhS~FByB5stYpwvGEqQZTS05w7La75R4fqngzi0Q__"

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Space Ranger v4.0.1 highlights

  • Space Ranger v4.0 now supports Visium HD 3' Gene Expression!
  • Cell and nucleus segmentation are now included for Visium HD and Visium HD 3' data with H&E images.
  • Space Ranger v4.0 now features a new UMI-based registration algorithm for Visium HD and Visium HD 3' data.
  • Starting with v4.0, anonymized telemetry data is collected and sent to 10x Genomics.
Release notes

Space Ranger 3.1.3 (Feb 6, 2025)

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    Download for Linux 64-bit (tar.gz)
    File size: 829 MB
    md5sum: 2cc7128cd538108d2a8a737ec54df484
    curl -o spaceranger-3.1.3.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.1.3.tar.gz?Expires=1770486664&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=LLKcUSjlrs5wAw4RwE3rRTlYNyPnexBo2TEaFWLSYSHlzJmMPKODo5BEXcEKqsXQNEAhmGySnsAMH3wM6y3s5BJfTCCpN-2HRN5bIcdKp-fYZYbRC4mJ-6ZZw9WQIsHyf-OjufSSI1Z1m7lYzBhdMcw1vdo~u4wU09iPnzYPv1SKYGj68z0g78Z3e6WxmxIrhAlril5mC-vgEr0bNdrK8xLGiY1u7SiZKU-qeA1Q0~ymvfW8fpO1gjZ-bRwmVOmXqkxRGTvDIVVUmHXNpLRfqUl-dAeQWq-wirWkckHDTInqwYiWJYoSwEABpOL2-W9c8GHV2ffrRu8m8AW7wOwxLw__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 529 MB
    md5sum: 2756aa7e3b66139059667117546c41bb
    curl -o spaceranger-3.1.3.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.1.3.tar.xz?Expires=1770486664&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=oJSjY-gXBMps6ILaZGD4qq44r3p~S~hA~4RvH4jGMJjSVNsJzekiY0TVcbQMiTWKvcrlxm56E-U-QRJMkD4SVBkp7JpRRi35mrNseJBsoUaP6Np88J~m9SRssXCdI9K87K5he6cLTgpHbkt0BIjYWEu8kZlBtPnbIddtGr-iw1r7sq6Dtgl7HmJIBNWOUGWhArWMRwwysFczhdua5gi3F4HNdoUVPMTLR8KbVsQFKVZ3FyYmrZB3XyyW4MCjXS1cqdY9Lj8wL1~QVoXdozSVoc614clJdmOFJJ~7IcexpqjeeJYA08K9QChgznEEtVk9f4D9Kwl1oP771K0eXtEJtQ__"

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Space Ranger v3.1.3 highlights

  • Restored support for AVX1 machines.
  • Other miscellaneous bug fixes.

Space Ranger 3.1.2 (Nov 18, 2024)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 867 MB
    md5sum: 949088b1e2cc5bd03079b60a65b41d39
    curl -o spaceranger-3.1.2.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.1.2.tar.gz?Expires=1770486664&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=oKNdaP1s6fKyQ3AK2iof6GJv6VeXonD10mGxxEPmTeq53FPgY4lYE2lRjzr4gAV0HCfiGt~Xb2RLFdmlWossFHvkqfKmtETa2ZH3cedQ-jNXnAEFXUDx2AbXq1cwqMs2o6ToUNdX-bdHovF~7dLHayCZnHge0-MRUwj28MTyOLX3Id1eRI0f~EAYf7i20~KaQ2mB6JKoiMNw1AgxM5QdsYRfA1QV9CnRgDX09Do~jX82o7DTzbV0fnHBIKlzUaLfSQF~JWED7f2Bz5TeEitAw0zQt~9E4yauDpFpCqY4DvBWYsTlL6vN0ZXTRfHyL2TiUEnXVzOCnyF0xpuBE1tXcA__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 552 MB
    md5sum: 5a923ae250eea2974fd1cef7b2f9c1e2
    curl -o spaceranger-3.1.2.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.1.2.tar.xz?Expires=1770486664&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=khKaL6a7JR-Fx4aOPjnmOFqUbWXpKd--bJYC5PdBpVYHxFc1mZRgZRBFHDmS55i3eM2kfT~YBXgVQRg~oN04bEv1YNvj7aBu9vpBWvJpGvhw94ioiyV~gG317yT6Xl2PJbM9qacYM-3eq3uvtcFfkWg5IxVpH6WyNY7niWCyIPOOvBaoL-7S5nvT0cLZZ6iQSjRYNx5sdnWw972Nofq47s7r-5jaqGr0x3S8bfPmY6tH6dmMkBQwp0qp9Wy-Awa37vocc-81il0k50oMsEPJDB8vcldOl9P9qlliZgW6GWNts-weOos0pPWyyblCmW7TCRMDUmkvR31E7UT76Qy7vQ__"

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Space Ranger v3.1.2 highlights

  • In Space Ranger v3.1.2, the pipeline automatically corrects Visium HD CytAssist images generated with instrument firmware v2.1 to account for a bug that affects registration between gene expression data and the microscope image.
  • Adds metrics “Number of UMIs per mm squared of Tissue” and “Number of Reads per mm squared of Tissue” to the web_summary.html file.

Space Ranger 3.1.0 (Aug 28, 2024)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 827 MB
    md5sum: 13a2c82adfb1f1efb737e2be121000d1
    curl -o spaceranger-3.1.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.1.0.tar.gz?Expires=1770486664&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=liOfpDjJcB3I3WinFEIV4Cl8~j2Y6e5LBXwfgag1j75b9q~ISVL74WfVjBtTb-zxIECpscPvgvwK6PD5oICL6x~JTltJMhwgx9uoq4~bxGQdguHD9j6~U6w5N9x48sP4QBTsWU7d5US08dfBHu6qjeSEwwX0ASVdvvchg~aALZNE9qijwjx~7zd9CJ1fbwQd74sVDcs8mBBLljLupeRZ-C687V8~ModzcQwOu7VLZoueWUn~-ymaM-iQcZ7lkgj0UIWPU5MHq-9ggGbSs-6jyKOInXFptLtcZCzy~NkGPIdRPf6j72EyzdEDpazvuOPNg7HwaoggcmUc8-omZw4Pog__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 527 MB
    md5sum: 28010d3724e9effc488470a961fe39fd
    curl -o spaceranger-3.1.0.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.1.0.tar.xz?Expires=1770486664&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=QA9wYC9b-W1fW~swf4Jfp~qZoQX2cK-oDNoM1OxfnfcZ3P7tDRPM~YG0j8ukVmVHI-xcTypWeZuKLtZTzPCaRugZfa6K0-FparEI6V5Yhs-fJqS6DQxXaz6iS0xv-hD1sDDPsHc9IM-f3lcPS9XtKh7NAvwsj06HzA1jiDbqZkcX50-ae9UPpN7UJozleI6295RDTkrTdzRkDmnZqAZlja6ICtYwg2eC5SImaKgvgyT~F~LVCn3S~c-qLDgSeS1h4AC1pXSfBpx92VskC-qOu5HMlD-O9AK4mXlfsDoRBKakFEm8Z1fOZMq50~K3eaEKYtV01ophZt5BxKvVn4sQ0w__"

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Space Ranger 3.1.0 highlights

  • In Space Ranger v3.1.0, the pipeline has been overhauled to significantly improve the CytAssist to microscope image registration process.

Space Ranger 3.0.1 (May 31, 2024)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 757 MB
    md5sum: ae65e7ffdddbb966277b7d1776f8c63c
    curl -o spaceranger-3.0.1.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.0.1.tar.gz?Expires=1770486664&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=RD5HDAz4jvm00nGeb-cr4KXXWi2L5cKM9~TphhzI4ukG-pkKzqoGS5xrnmz-9Pz4uQMgC6-SVOw6N6y7Qo-WsvE-gRNsPVr-h6L-3iPIQzlgaCZjrS8zgKvSgrLlf8LKdwPeV~IgAVNUFFY7~M7OyZ0Cn9GkL9q4KX7p~O1IzJqAAGnHoCOWIFfTG9LSyCggRK9j-~UsxpkUfmd0uW-OWKvvT2u3Qa4o5wz8JXEVKS2tKme4XacuF73-48tvuzQqpRqgZbNPebAnoyXL1CaynehwSe7ukrcYUYm6uXYkWK6pSM9FxXzbjyywfchtWgjDBXoSoC72KXHNABx~4SJP-g__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 481 MB
    md5sum: 451495ee2074ff9adbac49ff8851ea1b
    curl -o spaceranger-3.0.1.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.0.1.tar.xz?Expires=1770486664&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=BeAFVCnOqhF~XDvasxRZnZr~Xpkzi034QcRYs3SCtWi8D9tr2TPwVe5GaXQYYyrBTaFPAmOM7PNlFho4B77Dly4JN5SGFoblZsM~loIsJw8eec~U-FID8XchFCPJwvcB-Xi0Odoxngqi0P3U9twlUHsYmlPvKtmPFDZDDwEDF~UMmZPZ8CT9Ct8gaHluB23HoDsh7gY4NUDKC4pC47GGIddADUVuC9G9SXPuWPL~VHa2Ec~dZWKoRcPSRVJwULIEp8yaC2XFw5A6sdfTM10YaZJgCGH-9kJdgoCyQ9u6OQdk9v9UncKTb8eSwLTsJoA~V2n6qOPk6LIiyLPSyLvmog__"

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Space Ranger 3.0.1 highlights

  • Space Ranger v3.0 now supports Visium HD!
  • Space Ranger v3.0.1 includes bug fixes and other improvements.

Space Ranger 3.0.0 (Mar 25, 2024)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 795 MB
    md5sum: 19f4443f10cf94aff5768bd8968cb214
    curl -o spaceranger-3.0.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.0.0.tar.gz?Expires=1770486664&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=R4yoD1gCEbAKwgTFNpG9iPxyuKcHEi9uzLXjDMaHxOC1kSbXV0nVAC~M8mnzM17EBvEYhLHrCabKZ84X2lHBT3SMv-W87TDm7pyhyf1G-fNwBmV1A-3pdl78XAaiqI19A4sqYvCMntPs1KtNwVEEsvl5LAB7sIRqQ4wisidcZpZEso8R1SXFdVCLddGZ~OONArHYksCur7XoxPS3l-IyQvVB6ZoxGghuA1bnI0gAy8PDSSFYznfbtqTnAoqt175t6ken~iIqvInt5oJj75Ey4zri7PXo0K4rqUxqoZqx8Ahu55fIx-uhBI7sYla-~5HCmAO9w7VC3C-SwqCQaolWrg__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 507 MB
    md5sum: cd02b83fe849672066c40a1734819432
    curl -o spaceranger-3.0.0.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.0.0.tar.xz?Expires=1770486664&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=QHIUrD0u4fLecLlm9g5x3EsEA~QLyzXaAOEcF-OxN-3yfLQ3Sk-I0ZNjTx-Hx7N196EuiTvjYCLH82x~OeAZaAf69kS~ZZgrCyA3nXWWPrblx3WdisqGwtnHGqDrk28zfCf4D1tIXxPRxTYgC2zMX6NoURqL2Cqr54WdZ33Nrt5cNNqKrxAMCxdgMMixPC17vWInaBTRvLGibzhir9crSG0GLKD-ruXQ8JEWKzwV2TuxfesL2-CNYZzHBMB0ahGVCEywdklYCxiqSqsa6bQdocoQk4Oszh-X22C9GGJWqNFtb1R~81JuYEdOx8ERFlu6Bj55dBeYvLM-PCDgyaby4A__"

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Space Ranger 3.0.0 highlights

  • Space Ranger now supports Visium HD!

Space Ranger 2.1.1 (Oct 2, 2023)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 707 MB
    md5sum: 0ef64524af2735bd2a0fc9eef5a605f6
    curl -o spaceranger-2.1.1.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-2.1.1.tar.gz?Expires=1770486664&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=D2kHrBuUFLUGp84-1Lz8NmP3-eOC~Sva4OQ9-YKL0gACEVMntdfrB0uDverFPgwtWwb9Jf6PPbKtH~3Egr9SIXb9~bYp8~55~C-9ixohJO5MOaB7nDEkv0T8GwyGp34qmEbstGC5x63qBLOhAR4MuuP29XPoeSJG9JicvKOwWG5YuqpI2Yi6kuc8V-4A~zGgta0u4N3C-EOnFsml9INh3P6gEqbyABSTgJ5pq96mrzq4YqgPqiJ1UPjGeZC5857W0yPeVv2Qeb09qxuabsj-E5HijNcVMk~tnzqB6Q3Yhfg9oyFEO4fzuUkBhmCsQaewUU5a-lOqvbhvxbQOYg8NVw__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 465 MB
    md5sum: e1204bb11b3589bc5d5af244b51bfc18
    curl -o spaceranger-2.1.1.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-2.1.1.tar.xz?Expires=1770486664&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=XwCf8vc7alDsap0lunCAxZIAneUJlodvlhpll9yvEOpZWmXNA8TAeCLTFP3fKzCzDt5rZWCZCinr-gsDMH0kKFp5y8gnNNwLEaBGGfx5GVPBatSJ8AuCU4rezN4e~NrkJSV-gl0SHZh8nq8cbtIPhBECRtn89SG2c3T2l8DyAeufCaAzis7gBVjwa2~7S8oKolfhHXFq0cN35BfB8zRtpmOqZM2Ba5WXLrOmCkp1b3ecx31HHdxVFtXH0FvA7Y0bz8n63LOaEn~dznOOFGkV-NSTImZuCSSORKfU2ojrXMxjL4Ocd9mD8Xez-JTxd706S~Kh2IyGsDdwk3JBvZzd1A__"

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Space Ranger 2.1.1 highlights

  • Fixed a bug where 8-bit grayscale images were inadvertently turned into 8-bit RGB color images for tissue registration.

Space Ranger 2.1.0 (May 16, 2023)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 707 MB
    md5sum: ee94c453c44770b2b69d6be46b948e3e
    curl -o spaceranger-2.1.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-2.1.0.tar.gz?Expires=1770486664&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=Y7UEC2NmDuBDDdOfp2f4ni~ldY1AN66XSHavXU09~cASxRQn37ALouTxXTiofyxJOl2z4veKdl-3blNj9VQziUJc2tUQFeDyo-dPg9YBA8SC83tXrMNpkk98F8F26EoffyUhcIuyr~FEwyAGW~cZ35zmVN9hGAswz2~2TcWyCDrXL1fYGGCg4Ff-zDWuXMroCEvSrhgpL3HhmzpiuFP8wBp3i1OHDO1G7-kYKJ25LPaFPlt0Yo1qEgQldcqy4PVVRb6d4RlvP5SEZ3e7bIpPbmVPDsrrT4Ixx8xr47AGu9MBAVPqhGYpoegP6WO7WPN8JkTBxUB8tSnaDymU4drsdQ__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 465 MB
    md5sum: a0d90129a81c41cbdd728e23a4d25161
    curl -o spaceranger-2.1.0.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-2.1.0.tar.xz?Expires=1770486664&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=VStBNf7xWdot2ByVzGZFY99CUO75uVk4hSYkeMUc54UxyyjTjb6tG2B2p3FDZy5LuJgwSiA9aBdWgVRErqE8WLXX5U20NHu0lyuEN7KA-iBm9QR0zRJJYwC-9dToIVidVMYm2oQNp2LPZekowFRbcXNddHqi7NEjqqwxW1WTmwFjMUqStdXG7Abdx~KPL5Y5F5ZAXWo6ZPzX5JveAMNpL7qSeCntkZNIIZBL9aZLgTP5oAwANOwduJtEK8QnkK9UtDi4QiDg3WU7cbTXiMZbeJsMCHEYzDo~rPBJYka8e2s-O7HgE4Lk4RavreB9vrRoyLgCafNKb31YmQ4hr7ULwg__"

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Space Ranger 2.1.0 highlights

  • New feature: Gene + Protein Expression on Visium CytAssist.
  • New feature: Reference-free spot deconvolution.
  • Added support for bigtiff (btf, tf2, and tf8) image file extensions.

Space Ranger 2.0.1 (Dec 7, 2022)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 664 MB
    md5sum: 26151804338ae4d4b44151232bd64b50
    curl -o spaceranger-2.0.1.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-2.0.1.tar.gz?Expires=1770486664&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=VOaMP4rntkwDaII5LRp3Sr-8K1j3cOIb5ND2CqMbuEUAQ5SFEnYJdfOFLtPbI7acDwZexcEWFxZeuPkeOHDUDiJEYfMV2Hl5vXxjBfSoBUq1e3rU70w1PrrCGq1aqJWcWuKmKA8JIXhAhR45XXGRtF3zvtedqBOH~6Md0zz9tyFeeWhISx~iuEmq-eg9uneg4WNN52oYvY3SXv5uRELQUhlX5DLGmMkR8loPLP6bwG4wW9Uj~Vad4PJJpDGW3~G2Vk6t5h5dTHdM0fudUU2dIpUrXSqEwnIII0paxiaGy4OanbsY5p2pSL3o0NDSeS76N71-rv7vd~EpcNbXPecUiQ__"

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Space Ranger 2.0.1 highlights

  • Reclustering workflow now supports generating a UMAP projection in addition to the t-SNE projection.
  • Visium Image Manual Aligner now includes additional support for images with difficult to detect fiducials.
  • A new Spatial Enrichment table which allows users to sort and filter features by their Moran’s I value for Visium Spatial Gene Expression is added.

Space Ranger 2.0.0 (Jul 18, 2022)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 662 MB
    md5sum: aec9ec128c7bc9fbf9fd6d31aa390d4c
    curl -o spaceranger-2.0.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-2.0.0.tar.gz?Expires=1770486664&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=ZKPKNFWb6AQAMjXGS437Z9qr5csNDp3LKl6EVezVwcHQ8-sOv30SvBZCsfJKv9uF1X4icRsXKIWArsO3DC3oHPoLS1u4P9C392YHbYMMIe7XKUK2bjzSWkH5NfBNLT8QH8TsxB4Osa-AyQSUEONVTMaZVNAGF~I~jYIHIP6jjmkE-AlcuV7P6gnJZlQkfo8eRPwUL9FSaow6IXAUFRQNuzsS1ZJG9OYbC~6pB6--9DcDF6-K0I62RZss4mHdnGo00~yMP5riDgKFBjKd4PjhvEbDzfA0sCbRLf-WiQPpP01DNgLQJt9hTnmrItBi7qUnokYAMqJQCQDuckB0wToOdg__"

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Space Ranger 2.0.0 highlights

  • Space Ranger now supports CytAssist-enabled Gene Expression analysis and includes image registration between the CytAssist image and the microscope image of the same tissue section.
  • spaceranger count now quantifies fluorescence intensity in the image at every barcoded-spot location.

Space Ranger 1.3.1 (Nov 1, 2021)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.2 GB
    md5sum: c2eb73125d9ee731fa825ea3734be1b4
    curl -o spaceranger-1.3.1.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.3.1.tar.gz?Expires=1770486664&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=Q9lMkEoknaoaGNQowQZEnf13U7It0F5q8Z8Fxb2sX7P5pgq2wfr6zWV8H-~-5o8BgjV6TWkP9TT-q7Yczm2xsgutLOxX0pG4rXv7xsxjqGDguQkXPr0JBGTXtpBBKl59XXQs8noeZXRwTBqwKK7vzxGD4eItFH93OZSALHGDOFAyF25olijMe1aF6gHVh1DuboMXEu-oTxkzqQxIwK5LVFuD6rELl~fRSt4y~wOYYBqjoliKgKqB3HdQpOcvapmt3JG9G81dU6gITf0pgUz9OCnYCZxvF8NCzCjECBsjhqtt0G-A1qVM1TMBZhKfCV-jrglb4FP0jWO-CxIyVt01MA__"

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Space Ranger 1.3.1 highlights

  • Adds an alert if image alignment would have produced a different result if the --reorient-images option was specified.
  • Update warning for low fraction of reads confidently mapped to the probe set to be more informative.
  • Unsets additional sysconfig environment variables prior to pipeline execution, which may otherwise interfere with the pipeline conda environment.
  • Fixes an issue with mkfastq when bcl2fastq requires LD_LIBRARY_PATH.

Space Ranger 1.3.0 (Jun 9, 2021)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.2 GB
    md5sum: ede7f98db5adf7785e35ae0508c48f74
    curl -o spaceranger-1.3.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.3.0.tar.gz?Expires=1770486664&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=LjGd4XbTG7wBOcqKTnh0V0NpTdKQaW2IJ1lfm7CoGNgLKwPfVSo4A098TuH4kFuO4RWIRVZAxFz7kRc1vk7~1NJScPl76KyKuBBVFZIiQAQufs9wRb79PwrziLqRgDdbdxDaWwGOpvs2q0EYsCE65ajlihLurQ8SyQtXNkgaGZx56fFAIN-X8I6Ab6ZxSPeUTz2KoVapduuIiAtBdG9pdOLnw9OZpJKRtd4yGEHJtUaN3qhHfzrEk8jHTGpB7zg7g5N3-w6J~0fSbrapOSjz8qbgjLG0WSqFT8b0-gjRcWnlZsAlLOFx~ORTgGtYPVPVEYhe1-~ST~OAVnLNxAyd-w__"

Download is unavailable on mobile devices.

Space Ranger 1.3.0 highlights

  • The recommended operating systems for Space Ranger 1.3.0 are CentOS 7 or Ubuntu 14 Linux variants or newer. CentOS 6 and Ubuntu 12 are still supported but have been deprecated.
  • Gene Expression analysis from formalin fixed paraffin embedded (FFPE) samples is now available in Space Ranger and is invoked by specifying the --probe-set option when running the spaceranger count command. Space Ranger 1.3.0 or later is required to analyze FFPE data.
  • Space Ranger now calculates the Moran's I spatial autocorrelation metric on a per-gene basis.

Space Ranger 1.2.2 (Feb 3, 2021)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.2 GB
    md5sum: 3280a085714ce7c894dc14b76312bcda
    curl -o spaceranger-1.2.2.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.2.2.tar.gz?Expires=1770486664&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=glSrwy1WOpSZmYN6Z3pIX7YakhMfwBFQJoPIKnodrDxYbtTTv9wWkNXF5dFfZFnwFpDYgWcKjGMrF5IqpPQE6y5VyucirTHwRPj8dgzQ59njaq7L1arsF80IxdLJTegXq99CpqCbkdOGpyp0d5Tuh9RvMIimF6aRWbnzEshYvvZVgpOpCGHmC0jjZXvcx95Xk-DbkjPPoLF0DeUxiTVlBV59Ywr-dHX-mSakvAff9-A6iQBY1yTcEvXKQCaWpOXaUaN1oLNztkspzpXXTJ37GFpbwqFi7NO~xxxDXt0R865KeXq-hQUzzfEMojDD29VQfTowJpxBdGpUbv2kbpsU7w__"

Download is unavailable on mobile devices.

Space Ranger 1.2.2 highlights

  • Adds support for pyramid TIFFs, or TIFFs with multiple pages of the same image at different resolutions.
  • Fixes an issue where targeted samples run against new references would fail preflight.

Space Ranger 1.2.1 (Dec 10, 2020)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.2 GB
    md5sum: c24ecfc95c5e4f1c268354c5934da98e
    curl -o spaceranger-1.2.1.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.2.1.tar.gz?Expires=1770486664&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=dlERUuS6ktmEVThoDerj6uGnxBYGKgdRsFg~FqYe74A~QfxI9UvCnvMhIa6U37JAEFkZ9XAGPqqB4GAh5E1dcOlPXtAG2f1O7Dx0tiOOjvHL3buG4LSElJCiStopFih41hJfTrZRRaD3RvSHpB-paQBil-MDhSMaxeKYAK2ynyFLryvWimGBfKrTDBZyK7H9ovhOvpFRedkwdvjR9r~8LxgLO9pDTVATDiQXqT~EvpsxAXyTLd1jAurOMCQhv72~cFAV4Fupys6NoTpYMKznZvr3A6oXAdvYNAGBuzHFXH0~FlXhs0CtTRfAqzcMpYaNG416l-qZI3FYIqAKobARSw__"

Download is unavailable on mobile devices.

Space Ranger 1.2.1 highlights

  • Fixed an issue in aggr where files would fail to be copied on NFSv4 File Systems.
  • Disables multithreading in mkref to address an issue where mkref would fail on hardware without AVX support.
  • Better error handling if the pipeline fails due to a missing .env.json file due to an incomplete copy of the application folder.

Space Ranger 1.2.0 (Oct 27, 2020)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.2 GB
    md5sum: fc21902700779111afc3c813bbb0ff5e
    curl -o spaceranger-1.2.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.2.0.tar.gz?Expires=1770486664&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=USidz~mpN7Hj3ySOIZPOp9ICXmXX3mqNJ4llt2LlkHiUfIbVOOCcq5pqe2estdcyU7L2h3~JoiBiWX4LurCIDDnmP341PH5f7b5erta8e4cJ98IOyMrgTC8YqDxQux3dqBMajFUmSUSaevX6TacX2Aoqi-sK0DsRar5s04PzEZYpbi5PnjA8K6Ghxo3idC7EqOS-883AvFcZyPWn3w7Iy4XRc2UjFE5lEoqglpIwCBCnss2WupwwXj3QfYOqKn-KSLfpof7DN5ELdrN74TiNo9Mp-9nwaDiVOKr4oG9GHZqtw22nguds7dk-u59HvuZADkOFAJKCMTtOFY6r58QeAg__"

Download is unavailable on mobile devices.

Space Ranger 1.2.0 highlights

  • Targeted Gene Expression analysis is available in Space Ranger 1.2.
  • Introduces the new spaceranger targeted-compare pipeline for direct comparative analysis of matched parent Whole Transcriptome Amplification (WTA) and Targeted Gene Expression datasets.
  • Includes the spaceranger targeted-depth subcommand to estimate sequencing depths appropriate for Spatial Targeted Gene Expression experiments.
  • A new command line option, --no-bam, instructs the pipeline not to construct BAM files.

Space Ranger 1.1.0 (Jun 23, 2020)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.2 GB
    md5sum: 708b93997295db31bc457b3d9af79ca2
    curl -o spaceranger-1.1.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.1.0.tar.gz?Expires=1770486664&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=ma~n4HnX5ufFbm13Kl7Pvv4V87F-7SztYilTUQ7zEHoRIwUfEyTr170dwLtYqFvHrrnlNCrTVb4ZzRSGnAMRBfj4vDJ2GpF9kzuLyxpqHgDt-4JGLUpgNtarlv0d8b29nCcvO0cimKgYVHeHAm4f-1PhS8y2RvhAxSynu2fdJOC6vbVV7H~~wF9lLYfgd3BdlCCmkzAY5ocR-g~dBYJKIIrSEy-xJ83K4AiqaZi1eiSqSfMOk3zYioK0GClh8DiSGgAmCDnAkoyIbihPAyPIKehlzEOctf32pdwc1txK6kkgO0nsx884jLblaiSuneDze0pW7vUxsQSLsx2t5uJDoA__"

Download is unavailable on mobile devices.

Space Ranger 1.1.0 highlights

  • Support for fluorescence imaging input such as IHC/IF.
  • Aggregation of multiple datasets.
  • Optional automatic rotation of slide images.

Space Ranger 1.0.0 (Dec 2, 2019)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.3 GB
    md5sum: 5cc7fb86d0cbff7df687b11575efb7cb
    curl -o spaceranger-1.0.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.0.0.tar.gz?Expires=1770486664&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=lQhCcqmDKa0G5WCCPbb5~-ZCGDRYVN90oPOuByjy~ZK3DbHV59~xF65yBsqC~sMM25lj~KnN0i912S4y6xGBDtzPL9PRbUiZKc0LZUXVrojkRsatWAJj1KEo2mZC-VquluU5XX93xCKiBUClBfezNiakMZC6xOeb10GQK5o8RiAfS~eSrBYNMfrvu1v6QLCF3ypZ8YXsFAZNhv6fvNSXRwv-eN4qbRgCONjJM-bpBprt5X3-R~~pZ0hcmzrEu-Gz32mWU1psatjEpXxKHdjIbEUpmgyyFjxY6NvOGMH1SMetRttxtoNabnYZrv6YGMsxGaeormA7tUitSt-cziV2TQ__"

Download is unavailable on mobile devices.

Space Ranger 1.0.0 highlights

  • Initial release of Space Ranger.