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Previous Versions

Previous Versions

Space Ranger 4.0.1 (Jun 12, 2025)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 885 MB
    md5sum: b0beb100947717cee8c7ad0d7a9f0247
    curl -o spaceranger-4.0.1.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-4.0.1.tar.gz?Expires=1765088092&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=CjRseX6Bm~GsYmI8Ty0DssBSXPEu5QX14nygP-r2xeZezqWsxuc4cwWecVYQmhkkEmpLBBFozIz35aPgZSOeYJxzbwAFW4cfSlgLUp5jpz9hdwg-nC09VKVqSH722ADiskV1JnfEVdSkCPWwBVED4NtAOnfQIY3nH8LbHp7YnotHqzNq1dnxrFb3c6jN0I6vQ5AaiubkZEGdtVc~5pZjVHlHdn9B3wSYicMUdl512Fk1nlk9t2K6B7sFUs2UTzdXeCvLWZdSY~1dZB96R46o~HfvCXafFrH8iU1FZeB5B~zHqf49GvNgSd6VzHsW-0yjWvz1vfwtEaeADEY4hGl4CA__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 582 MB
    md5sum: 3610ff948a6f8129cf49509f1bb34970
    curl -o spaceranger-4.0.1.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-4.0.1.tar.xz?Expires=1765088092&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=J8PYnj8c2CW4~knJAZZ~O-ZX4AmBCnGcXRPqWDGsORmSCiNUjdvMeBaeHhf8ZzCRSOOFx46c0aPwUX0L5KdmPf-BYkns1HJQmqUT9YHkcJDXzx80tYJy8rSMn8JiWtv2MHm52MtDI80RmssuGelnoN4AvgiEjMDH9SBp1eBvcQ4U7-Xda10rfh2at05jlN4A~wUEMjjBpUB-Ye5t6TlVCffLxJAM-CPdaUFmJSdr-fgFiltPIhE6hjNxcx8agC9ck6EwK8f5YT9fH9Y7AFb4fJs~dqs9irvfw9S-lv7X3tXjZsYsmkjFy-bV4lm2n7TGX5~dt2bcbvSFdpYbCUpAaA__"

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Space Ranger v4.0.1 highlights

  • Space Ranger v4.0 now supports Visium HD 3' Gene Expression!
  • Cell and nucleus segmentation are now included for Visium HD and Visium HD 3' data with H&E images.
  • Space Ranger v4.0 now features a new UMI-based registration algorithm for Visium HD and Visium HD 3' data.
  • Starting with v4.0, anonymized telemetry data is collected and sent to 10x Genomics.
Release notes

Space Ranger 3.1.3 (Feb 6, 2025)

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    Download for Linux 64-bit (tar.gz)
    File size: 829 MB
    md5sum: 2cc7128cd538108d2a8a737ec54df484
    curl -o spaceranger-3.1.3.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.1.3.tar.gz?Expires=1765088092&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=H7jdmw0NqhMxjGmmYQnVPLiJuWhMwGF6FjaC6QNbNkU9fN9959g52m39XMEKuIT91knyBoKvlIQDzLe5p4ZQ~y3UBqGSpp02jDsYBDnp8XSreN7iZFQUEu16lmoRMWj93AEvkPzCStSZv56LJEtVa46iVH-GsZVNGi22CvNPWbcj8Z12Y~oRCoCOIVTEMaPneozvMfICLGZndoGXD6cLKWWTZfU~H-g0NrO~0SJxSIIDTVHrcFPUcB3IxjNefw9FnPHFCItpFCzHlO1ZSVquZTGO0FOovDyeVrBZHof7TVOeJ5-LWWs7A5B7Bvg6bjcQU5BYzxiv7BIUiLlZBozVFw__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 529 MB
    md5sum: 2756aa7e3b66139059667117546c41bb
    curl -o spaceranger-3.1.3.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.1.3.tar.xz?Expires=1765088092&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=ZzUGdiPcfyO9cIMKnK7DV1WJixuJm5hSyPk2TP5FhXGHBUdpnnTHIZ3as5rhus~nnOr~K5RF1oJJaQM9R~feXun4a-6jzam9z1Aezx2xYBlnASLeGurAyC7xzviyTXwl6wBMX8BzQHKvb0uHET3Q2bXLWX0slJTUBc7ntvBkRe2AwFRwjkRXg4z3gttuDM-CNxvlDoWt70JSOvzqxe3BnKpUB3lMPOGvB0Zs1OR~rMevIdmx~LZB42toPfLGaZz6F3CxnqM6SMY17muETIRaTF7Vq8KgpBA8-Hy4wjTxIFPe7jdqROb2szguat2Jl9Ilvun20kJvSj602wr~MQygiw__"

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Space Ranger v3.1.3 highlights

  • Restored support for AVX1 machines.
  • Other miscellaneous bug fixes.

Space Ranger 3.1.2 (Nov 18, 2024)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 867 MB
    md5sum: 949088b1e2cc5bd03079b60a65b41d39
    curl -o spaceranger-3.1.2.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.1.2.tar.gz?Expires=1765088092&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=QF4fYj7ndgLagJuFEnm0zLBPgkQLyHE6eVEXOYiDPNbsN3lcy-NwM9CkCNsRzWP8YlsFlIB~yEaDripmq3OdqoKysoYpn-Y10ZsOKd-InblLL3Hl6fGI9Ey1Oxp-TcP7hKnUtuEA9iogrHoQZuzfrNv9hsXHc3PyyvMWUxRj3QAkB9CtI-ha187UMnANxbDsDmpOfNpsJBDwcqfrUzDEoTgKST3uJ5XAn~bSraT8knscBAba38Y9vshPt3a3RDNIPJtJ2WM7uynyKeTFSWT4ZT-dgNQ9gYj9ngYxWE6fXaFnKEQ~2KHFsCv8-Bxge~eNS-WVnlXhB3iVreYi1TfaoA__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 552 MB
    md5sum: 5a923ae250eea2974fd1cef7b2f9c1e2
    curl -o spaceranger-3.1.2.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.1.2.tar.xz?Expires=1765088092&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=nIg~8xT3SXQtdOU2YJoHcbKPPqyASc9EAFJzhWTjBLwQglOhfGJt4nzWtT7kUmF4nroiFR6Lmre~KTl4DXXbnaVkS707rl-jcDHXThQKrgTayzKGYn08eV7JUpkhNM7XBGUzFkxKrMWZcREZH-lhYKNZdrKoyzWct4Mo1C8bKQoUyqcNiIf5M3Dn3~wRhD1lJMtyjaCQ6MmRyGuQt2Y62tCx5XVeS~SAq77jcFK~bqW2UChQxjOG-gjeYnov1ci4Um50CouZcZhttZAi-XTXcHNK8HVi4fbE7KhDWZXYBJRMP6NpQtdU-YGM1Kl~r0PjH-jRUj0~ocRitC-bLXfl1A__"

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Space Ranger v3.1.2 highlights

  • In Space Ranger v3.1.2, the pipeline automatically corrects Visium HD CytAssist images generated with instrument firmware v2.1 to account for a bug that affects registration between gene expression data and the microscope image.
  • Adds metrics “Number of UMIs per mm squared of Tissue” and “Number of Reads per mm squared of Tissue” to the web_summary.html file.

Space Ranger 3.1.0 (Aug 28, 2024)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 827 MB
    md5sum: 13a2c82adfb1f1efb737e2be121000d1
    curl -o spaceranger-3.1.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.1.0.tar.gz?Expires=1765088092&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=Ka5EawyB6pBMEpzddPc7Dl9gvliC-Ikh4RrhSd-g-yGvpG1tRFHqGVIWRhgZWctvmUxe~X48ZsOlLmqGZl3oUV1BMPh2fDusM8PqFHupWegRNhh4IM6JeXyXF67-gyx5yZ4IBeZOUBqQ5-PEdPklQ9VZU638-5WrjZw3jeCqcvFpEQ2VRfg6jA7jYIiDQL~btG9MMLuboanHMHsr7GJ2yoV3-xWlIUQrnmF5kC6SthqyQqeaQU1WeJXoSCaLDCdFpcOXrSUPNpSzyUKDEwPNIj3vKSmgw1VzAGnG0oX-jkSAj4tEPcNy8TCApyB1Dlqv1xW44QYFJQ1b6o-C4wRK2w__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 527 MB
    md5sum: 28010d3724e9effc488470a961fe39fd
    curl -o spaceranger-3.1.0.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.1.0.tar.xz?Expires=1765088092&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=MkZ8I9TVj5vGnh1gZj4Y-ApUI4kz2Lz8zmN50FYLRxVoIEUhwYzZrXRFC7qWZIjPZtxvvrzV4ZE7PqRnHZzlvSkfkfZwAHl36lQFZp4Np4mIeoptSKopUtHDnVDQte523NDrUiN1vERcFCLk5vuCrVu26iI4kxnoySOdxCVf25IRLTBIxXxigIrC5j7OM~ih3FgCkJkGt0cv5lND76ktE24YWJkZoUjSGbfiifQqpkdPMOB2bCrVShwmnpRpoF~QKk8j2r9rdQYbu0PmZXUP-PT8whg9IsyZV3om0a-hpIMse09vJJ8ObRITmvFIpY~M6W3X2Je6G1NIM0p9BMoVVg__"

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Space Ranger 3.1.0 highlights

  • In Space Ranger v3.1.0, the pipeline has been overhauled to significantly improve the CytAssist to microscope image registration process.

Space Ranger 3.0.1 (May 31, 2024)

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    Download for Linux 64-bit (tar.gz)
    File size: 757 MB
    md5sum: ae65e7ffdddbb966277b7d1776f8c63c
    curl -o spaceranger-3.0.1.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.0.1.tar.gz?Expires=1765088092&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=C6D0GmvublBh8E0Lf7aOkJ3Ycm3lgi928aSOumVxs-9KM4IgMA7pIUTRwb815ru4Hgv-fUY5nQ-sVujvqTt~-iiR1jLnbynCMEWjfnkFz2QnX~J7vIO7w4-Z8OFljAoIwAuKqtMq~bUIoC8aRQwxPXYtu7EmMh-F7fkEG9MJFrY6pkbkaCerEN62ocWQ4dqhK26yhEnGKOnT6Ejd5UbH-fZlI~lYOkf1Okg~0Vh~5JtGDfLRt~PgKTJ5xRp33K5EKpec34i5ttnvuYSs~BozFUvK-pmjSwUixOJThKKnpeUWChcZeqRxKtXxWyrNoWrx14FeUJw6m1bx3gcZngEyrQ__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 481 MB
    md5sum: 451495ee2074ff9adbac49ff8851ea1b
    curl -o spaceranger-3.0.1.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.0.1.tar.xz?Expires=1765088092&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=VSzjG7SCoHp-j9GhNT89NpK0o-ebxeR8qcst10wLAOHdSY8X3ilqHYlN-sqCWpkKfGGDmHwjr6JvAHLoHLh7~GB4YW7vlywVUZfbOzVs5TM5yqAVCfm0OcZfiuBKkjy1TetNIH1GWLDS7yQLXvL-ZF5XlGeJ2SSVIleAIlrPRo-twFWi424yYgGVz3zugrPPdL89DyxxSmX0fDLmrQi8Ah65Tp7Dq0awi2-OBPLcVgKaXg4k-oup5oOeCf8NQCBiuBEfvtvw6NLP3ZhL3UdyBY8570xKc9ZkctXPG4ZOazyQQwtFWe6CoMg3oOoZ4s7~6UzY1Mj8o65Ht4y7Z~oxdQ__"

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Space Ranger 3.0.1 highlights

  • Space Ranger v3.0 now supports Visium HD!
  • Space Ranger v3.0.1 includes bug fixes and other improvements.

Space Ranger 3.0.0 (Mar 25, 2024)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 795 MB
    md5sum: 19f4443f10cf94aff5768bd8968cb214
    curl -o spaceranger-3.0.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.0.0.tar.gz?Expires=1765088092&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=VWBFf1AUQRy5z3WsHcsF4e1l7JmHDVmbt~ke~ZNYeZsjL7et-AVl25dNr~BagLZHTAxE2b~xRw8dsGZpvikqUBoVuMFEih0ic~TX9RNvimkf0IvaSvJERM0nF5nIdBVFawXNy8Ip1lBcww6YENojZM~P-uK493aPo-f~MUAuBhTaHteDdROqQ~IDqHtEFnHpfUxvTkjhzy~Ypnkqa~2KvbwfY5OYSSBuGlfnU6l2pGb5XCC0LErnAZLVZFONP7dL-4xYCnUV9Anvd8yexpk2vHPJpl2APyD6238qAKglk0cq5r~AYpHhXwu~2CMPtjXwM1LAOQJ-bjrqvX9U4XoRPw__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 507 MB
    md5sum: cd02b83fe849672066c40a1734819432
    curl -o spaceranger-3.0.0.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-3.0.0.tar.xz?Expires=1765088092&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=DIeA28ehPJkFAGLxTkStY7Y-h8XciQQJPcFg3GiMNin0UwWOLdtuMJPs9rZ3amJHXCev-j5azM8Eb4jWiWlWmuL5WrdDn5TXNilE7thOfH58fP53Fxc6L~v4uHH6iiIxpyuo4R-tIqu5aAEGdozl1GMUj8xwp9BX84OheE5T5T9ful1Cr9lWdcDuIqcmFUENmAEwSaqQfvjqkFvYZOzzKVsx0a-05YMr8ABudIqwcsLfKAYiEsnTr2bDbdgBer-SdLSzDM4Eew4k-HvksioQKjtxariB0zgz9BTKMQIeuwPQA1ESK4gXtzUltKA3W5y5QMCfnjk9LEA-~n-tn~DtFQ__"

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Space Ranger 3.0.0 highlights

  • Space Ranger now supports Visium HD!

Space Ranger 2.1.1 (Oct 2, 2023)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 707 MB
    md5sum: 0ef64524af2735bd2a0fc9eef5a605f6
    curl -o spaceranger-2.1.1.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-2.1.1.tar.gz?Expires=1765088092&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=Fw~ifyIYXQOOmJqkNDLQPps0hIV7H4ozQPjocOS0jNH~8C5PxkWKbLu7pfQ3KTTuj5LH52zSKcN1eTEkQSxXcaiAD8txnlitxE3AStBGkef2a7ktxO43-yEvZw6dWlRHskpch7d9aDe-d0f4yuF4qQUJ-tLLqI1qMRHWJT05sGn5oACqvO3otdcgbz9c9rWzAr2nz0-DBv3raKtndcqNHuUAKPOQHbgpdSOVXqwcD~V5z5Ju5x7iT02UNFNIC6XDcX7Drr6eiqnimvVDNYJWOeA6biPndLP9ldXjb~mFauDWm1FzrWQCoTK9babDjApy5i3ovVzdlCn1rVosJBiSTA__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 465 MB
    md5sum: e1204bb11b3589bc5d5af244b51bfc18
    curl -o spaceranger-2.1.1.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-2.1.1.tar.xz?Expires=1765088092&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=C2oMZfE5kG-z0T1fVHUQAkLdPcHypa-IskGe7PoRk4vUX-aw6ehYy9KxZVcCtCcNuvZ-OARYXh2Z82-nF-GaSqlkXjGJ7atFgtAlYlnWBIU~Z1MTlBvFHyD8g3lr34XkSiXFcrPahFGLYWDEv-o2rWtvOe1yERk414FuMFiMVVX3NCTu9TX8YtuMJ44RvjI1b~tBD8vh41NDqR-84zFVhsGCH9wgVbT4-Bq9ONFh1SlwAYNT6Na6gDaUUa31EBF0Gdl~6i2Tf5W~oca0Rr2P28dGNwJBexDmfaqisUhBSXhRi56KH9hRWxT-2ZenTgmOb9abnIWZUOQwLfdCiD5jWA__"

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Space Ranger 2.1.1 highlights

  • Fixed a bug where 8-bit grayscale images were inadvertently turned into 8-bit RGB color images for tissue registration.

Space Ranger 2.1.0 (May 16, 2023)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 707 MB
    md5sum: ee94c453c44770b2b69d6be46b948e3e
    curl -o spaceranger-2.1.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-2.1.0.tar.gz?Expires=1765088092&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=meTOWA4luOcd7pewXtTxwyGDcZew4eMQnf~uWl~Gfv3xN~exfelaASKvsmADSa1bSKjr4IldbOASJDGFlxlBI9YLSoRJSB4JIrpOytNu19Wa1qY9xZtdn65bwFoPOfLovwwVoilFKukkkgZSWLr4HqJNkNTbTGosgHtau-mQ6nGQOnpD7uONonN~m3V7ojB1vLRTCxpzIZzRqQXQJtFHKvYyPhZpYOkm2Ylfq4nfiFDm6-BhqMi5spxeRT-wekSRuVJqNHzHbUsuiAjEVdYv16PnuSaflT409PNfwlKBLPfQ6z0QvOEai3i3CU~3j9ZK2~SV2tssg60Obv1IZthrjQ__"
  • tar.xz compression

    Download for Linux 64-bit (tar.xz)
    File size: 465 MB
    md5sum: a0d90129a81c41cbdd728e23a4d25161
    curl -o spaceranger-2.1.0.tar.xz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-2.1.0.tar.xz?Expires=1765088092&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=V7KAlZ32gwxCkkd9D-PH~dbov~kKGko6TAREgRpWWWisM~slYCA0u0DN2JWhai6EODKHZ0KdY6sd61Xb2t-CMPv5w9vQjR9LWiiC9oo-3kPa7Axe0pWPgdSa9jvqXCI3t96TlZgZ95tMYdq1TN6bRSJd-8d~5v5Yo0in8ChhyooiI8CqmdU2KqjSbN7nm7sFOMEnS12DijJiUrSW2bYVpU3gHCRmz5WFNmeTaGOE1oKe~DhNaZbes6~Zt4Jx7qz2yz9tCFb2eCUq4MsLPrZEg63S9Jaimce4c~QuEVM5~4Vl7WaZaizQggwnHqPeI6peJWKgV0eQFJ9458hjOmCJUg__"

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Space Ranger 2.1.0 highlights

  • New feature: Gene + Protein Expression on Visium CytAssist.
  • New feature: Reference-free spot deconvolution.
  • Added support for bigtiff (btf, tf2, and tf8) image file extensions.

Space Ranger 2.0.1 (Dec 7, 2022)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 664 MB
    md5sum: 26151804338ae4d4b44151232bd64b50
    curl -o spaceranger-2.0.1.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-2.0.1.tar.gz?Expires=1765088092&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=gGIvVLN9kjq0VHIR30eab~E~KTb5rJq~tZQTwyQkMjBbaFf8kUWsBxVBP1kpe4hHWkdv~WRHbizbnH9fgKcxPB~VOCm0eA5CK39A4hlWxlgs2goJ5SdAfEJrOIVQmZeavkqtohnrzbsz8K60hjPTg0v9j7cqMpQPeeqQ0U5ckCcbZJJ4plrXJ7mogUHP~wK0r9AEa30VvUqrbXt2ogtSSlnmwj7KjUBioiuEObJFZqTGeKE0UQMKJoBovUrCRAUUWy4v46KQUthhZNRlJgsJI1rCihsRqEw~u70peTmpUJ6wN6iDYcWmB313hjXFmgEccYDuuWw6Mnrardy4oz6aiw__"

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Space Ranger 2.0.1 highlights

  • Reclustering workflow now supports generating a UMAP projection in addition to the t-SNE projection.
  • Visium Image Manual Aligner now includes additional support for images with difficult to detect fiducials.
  • A new Spatial Enrichment table which allows users to sort and filter features by their Moran’s I value for Visium Spatial Gene Expression is added.

Space Ranger 2.0.0 (Jul 18, 2022)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 662 MB
    md5sum: aec9ec128c7bc9fbf9fd6d31aa390d4c
    curl -o spaceranger-2.0.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-2.0.0.tar.gz?Expires=1765088092&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=LaOblRybs4CZy1yCwjoVVCy5DwydNCKCwNzdH-D3oahBZQTEBdIm98HGzhW5DVdAlwvletF~t6WKR6rCcBllbTTdoGa3BVhKXpYtHA7ll10S2Mbv0~PNAhQSpBVmjue~~dwINxSk18nIo8re~wa5wQuGtd7dMmUkQLyx17hBwRrI1FWiaf-dFl3TfalJFh3XYu7hdi0KZeugutP6AIkwytqg-noixOLQI4KQ3ermUTQSptAI1gvnsxZZj5j8JneWlB5aniKDC~x8mWuQy0TqBFdd1RKX~gJMBiruOCkoaDt~Vuc53XFoBgRWNz9D0pxNbYdfGqKF4HoMp~IvKAInIQ__"

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Space Ranger 2.0.0 highlights

  • Space Ranger now supports CytAssist-enabled Gene Expression analysis and includes image registration between the CytAssist image and the microscope image of the same tissue section.
  • spaceranger count now quantifies fluorescence intensity in the image at every barcoded-spot location.

Space Ranger 1.3.1 (Nov 1, 2021)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.2 GB
    md5sum: c2eb73125d9ee731fa825ea3734be1b4
    curl -o spaceranger-1.3.1.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.3.1.tar.gz?Expires=1765088092&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=dL17VhuoZTXCMsQZvywlLjM5Rw6vIsdkUZcTAvMnxg2unpJK8wiF0nqwJsud9PpokSeMC~9kBO1sZkINptZhPNCX34TvnLbI8lpL6Nvs-EAVv9kSpqYFqqT66P71ubGSorCYXTlorUBRaSI~cebTWgBnhMVNp-lMbFMoqNiHDhAA7NypdlmAUAY5jwuFtroACKg591eTZy45TiUzySxMAbkp7ipi9OxGgXscMSvcNcZ6X2SbSiKdpZiHpOLyOD7NMuKdMlImkXRhFnJIkaXkTW~HisL~I~zIvRrLRCCMRSrFK7Trs3-EQU2R-C9QluxMZVI62JjD2CFAzbX3H8V3Fg__"

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Space Ranger 1.3.1 highlights

  • Adds an alert if image alignment would have produced a different result if the --reorient-images option was specified.
  • Update warning for low fraction of reads confidently mapped to the probe set to be more informative.
  • Unsets additional sysconfig environment variables prior to pipeline execution, which may otherwise interfere with the pipeline conda environment.
  • Fixes an issue with mkfastq when bcl2fastq requires LD_LIBRARY_PATH.

Space Ranger 1.3.0 (Jun 9, 2021)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.2 GB
    md5sum: ede7f98db5adf7785e35ae0508c48f74
    curl -o spaceranger-1.3.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.3.0.tar.gz?Expires=1765088092&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=ZlU8nOsH3a~dv90eqbT4378DSfiDezgnQS1bE3VgZjPfW7HjlisxtJHJyzAvfn0nTMxCABJ8uj1QugRdeivSRCX19vuCrRCSbrz8DQHaYm-4bgQzJQKeK~Oz86MgWQK0-bfvyHHdimZPNgaBYxoE3v5XihZuuNwgsmvRKLqhO0pqPP1ssLD7lJEzcu9VH9hxrsTnYlqIdc~MwGTRIl3Gp-zlAvGjT3VCnu9O37N6DNmXLfTCsVzPIvO5PYkOXkME2AUDu2iUiV0ez~jk3v7sH1p8Dt2GaTq0H0UJtuR7tlXENVWgIcam5p9LFu~9VJlx17Nr~0XZku7j2RvLznjEHQ__"

Download is unavailable on mobile devices.

Space Ranger 1.3.0 highlights

  • The recommended operating systems for Space Ranger 1.3.0 are CentOS 7 or Ubuntu 14 Linux variants or newer. CentOS 6 and Ubuntu 12 are still supported but have been deprecated.
  • Gene Expression analysis from formalin fixed paraffin embedded (FFPE) samples is now available in Space Ranger and is invoked by specifying the --probe-set option when running the spaceranger count command. Space Ranger 1.3.0 or later is required to analyze FFPE data.
  • Space Ranger now calculates the Moran's I spatial autocorrelation metric on a per-gene basis.

Space Ranger 1.2.2 (Feb 3, 2021)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.2 GB
    md5sum: 3280a085714ce7c894dc14b76312bcda
    curl -o spaceranger-1.2.2.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.2.2.tar.gz?Expires=1765088092&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=nCB-gDD5XMHDdTjK5eSXjdQoBz-3eQu7xQauJPcYO8J7D2htlBA2NPnZYjwn8YSrWUweDLYKdXJ5lse8ibB2-uJQa-6x~vt4UMlBEDyJBhpWKUYGIZSeJVc1uq7NmzF-H8Md5aYTN8GJFItFttSobkjM5wuzDplyfn3YVtp~fs8-oAZnJtQVyq0Fjic6kDMG2zkitmLsZBCrNjDHdIrWOlCrX2jx8w3dPi8Sw-q5RI01dzLZvRlrBn-hmn9Br9IfiMwdc7jA4It00cHwZiAQLhrfpeH8bhCpa-~Ww9uqxV9faxUC~49lXIh22y3kZa9SV07fcQi5CahdXqm5QhE7cQ__"

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Space Ranger 1.2.2 highlights

  • Adds support for pyramid TIFFs, or TIFFs with multiple pages of the same image at different resolutions.
  • Fixes an issue where targeted samples run against new references would fail preflight.

Space Ranger 1.2.1 (Dec 10, 2020)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.2 GB
    md5sum: c24ecfc95c5e4f1c268354c5934da98e
    curl -o spaceranger-1.2.1.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.2.1.tar.gz?Expires=1765088092&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=YIUaVKu7ZxYQj6FC8tHTd0N3hXDTtH2mm8WJHqkB03KrvzdMOnDAKLadmNSw2AjGgXcQ0Zs6IP9hWy37y5bU7W-AXU1rq1Pxz9IDyTLW3DDvzYHHVeUn4bBIN6aV-D4t1mzNJBMPO72UTW1PBOBwvaTUgKxZzvaOyoX8o3BtmBCLdLD2yLpL2Fgr5Ge76BCGYKZbBWMumWSOUIt5QtMeJesYhnyL8yGoLaBodL0bTk2zDOHEfnSaEVo-StRqTWqnLDU2k10uX2V6AGwZQxBTGLanbFsi0N-YCC8gykhK98vsO4xrYH0YjmTn~CkS6rAnJ8qxn8Dp-cb1Pmp9tqNwgQ__"

Download is unavailable on mobile devices.

Space Ranger 1.2.1 highlights

  • Fixed an issue in aggr where files would fail to be copied on NFSv4 File Systems.
  • Disables multithreading in mkref to address an issue where mkref would fail on hardware without AVX support.
  • Better error handling if the pipeline fails due to a missing .env.json file due to an incomplete copy of the application folder.

Space Ranger 1.2.0 (Oct 27, 2020)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.2 GB
    md5sum: fc21902700779111afc3c813bbb0ff5e
    curl -o spaceranger-1.2.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.2.0.tar.gz?Expires=1765088092&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=Oi6~3M8uxMQuCM8krSL6k-JWfkAnlUsTn40Olb89otIE-mPUoG~9DWa7nzZ1mkwgNAmxhqdVLPhonieeoG~8bM~aJEsgRpGa-AyI0O6K62Hl0MP3cDYkzmtloxYayjgpZSWcXYKmbNLu6awTcSFcwqy2KJukKVF9c~x3xD~GQAD67HAwh0l5soLY0dMmUxIqvRfZIeWWteGsmykZ~0MjANPsqax-O9bJtwKVOUdRLMcB8emx-3r7U4hL-Dbh3WUXsCRj-7UfZ507ebJ1ql9WlhKk22-PgvOkeH2Apkk9yscmJGibKovn7X0pR35RnqeBC~UU4gBKkmNwoSIHNyVfFQ__"

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Space Ranger 1.2.0 highlights

  • Targeted Gene Expression analysis is available in Space Ranger 1.2.
  • Introduces the new spaceranger targeted-compare pipeline for direct comparative analysis of matched parent Whole Transcriptome Amplification (WTA) and Targeted Gene Expression datasets.
  • Includes the spaceranger targeted-depth subcommand to estimate sequencing depths appropriate for Spatial Targeted Gene Expression experiments.
  • A new command line option, --no-bam, instructs the pipeline not to construct BAM files.

Space Ranger 1.1.0 (Jun 23, 2020)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.2 GB
    md5sum: 708b93997295db31bc457b3d9af79ca2
    curl -o spaceranger-1.1.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.1.0.tar.gz?Expires=1765088092&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=Gfen43ZxLtpQ3Tt0V6xYlREkaWnYNDXQ6dfz3hcsU~V2uXq1WBleU4LZcjgFVMiF63n1dhtPGbAvZEFiOYqOP42XMVF0ufAWgYP78os9mH2j~VnCL2NpI9gB0PsnLDypN7GeKdrls-exWDO7H6Qj2HUU5DbiPc3vpZ1bz4vL2sI8A7K3DrNwcIpCsrTgP4MYx-66NIxmTSqssDxgpl5P-CaOIYrSgmxj56sLSF7QrzU6hx73DjlogIkT289jnfYMgjNWzk3-EO6vP8LvdSlKGe1jackLhyD95TpaXFTg6SX-BAFo7KTmAMVKXOBRfuOTHcTXs3lpKi9~mOuWQDyJpw__"

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Space Ranger 1.1.0 highlights

  • Support for fluorescence imaging input such as IHC/IF.
  • Aggregation of multiple datasets.
  • Optional automatic rotation of slide images.

Space Ranger 1.0.0 (Dec 2, 2019)

  • tar.gz compression

    Download for Linux 64-bit (tar.gz)
    File size: 1.3 GB
    md5sum: 5cc7fb86d0cbff7df687b11575efb7cb
    curl -o spaceranger-1.0.0.tar.gz "https://cf.10xgenomics.com/releases/spatial-exp/spaceranger-1.0.0.tar.gz?Expires=1765088092&Key-Pair-Id=APKAI7S6A5RYOXBWRPDA&Signature=YX4m8G0po3wIYZWdX-JWfRGm7Z9LY1nLQoaatmFJ0J5FGDcfjDd7zJ1hpajW2z021xIoTh6YDLrLNVonNczX5xw-LmCOIezoRUqP-klvdKzcc1tnn3ULw3Z-UrNRvHO27mqcbnewjY5HeKvs8riLKFCo-UPMpeQJpyuZJXZuUMESDF6qY5qEF8sXBtDCtuMJiIbXLxpqs~30P0n79DghgwH3B1JaUkAeLr1k0LAeEzbnu5i2e55oLLZM2IlZNzqSgu4IZWS~5LGeybVydYBD3YRTl6zfn-hDNApm6dZZt7RZQgrv2imIilk09Pjr82cEPS0bm7Deb-mEobcrdTtjIg__"

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Space Ranger 1.0.0 highlights

  • Initial release of Space Ranger.